Coding

Part:BBa_K1969002

Designed by: Yijie Lai   Group: iGEM16_SJTU-BioX-Shanghai   (2016-10-05)


Homo sapiens adrenoceptor beta 2 (ADRB2) with 6 x His tag

Homo sapiens adrenoceptor beta 2 (ADRB2) is a G-protein coupled receptor (GPCR) activated by adrenaline that plays an important part in cardiovascular and pulmonary physiology, and is one of the most extensively characterized members of this large family of membrane proteins. While the natural protein doesn't contain any mark for us to visualize or quantify its expression, the 6 x his tag is for immunofluorescence and western blotting.

Sequence and Features


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 778
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI.rc site found at 364
    Illegal BsaI.rc site found at 562


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